RNAseq of Contarinia nasturtii: remaining carcass rep 1 - SRA

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SRX24274238: RNAseq of Contarinia nasturtii: remaining carcass rep 1
1 ILLUMINA (Illumina HiSeq 2500) run: 32.4M spots, 8.2G bases, 2.8Gb downloads

Design: Total RNA was extracted separately from each tissue type and replicate using the standard TRIzol Reagent protocol (Invitrogen), and then further cleaned using an Illustra RNAspin Mini Kit with DNase treatment (GE Healthcare Life Sciences) to remove any salts and potential contaminants. Total RNA concentration was quantified using a NanoDrop One spectrophotometer (Thermo Scientific) and quality assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Total RNA (1 g) from each replicate and tissue type was sent to the National Research Council of Canada (Saskatoon, SK) for RNA-Seq library preparation and sequencing. Libraries were prepared with the Illumina TruSeq stranded mRNA kit (Illumina, San Diego, CA) according to the manufacturers protocol. Paired-end, 125 bp cDNA libraries were sequenced on an Illumina HiSeq 2500 in a single lane, resulting in ~27 million reads per library.

Submitted by: Agriculture Canada

Study: Swede Midge Transcriptome

PRJNA1101052 • SRP502072 • All experiments • All runs

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Swede Midge (Contarinia nasturtii) 3rd instar larvae transcriptome

Sample: Swede midge 3rd instar carcass after salivary gland and midgut were removed - rep 1

SAMN40984126 • SRS21040197 • All experiments • All runs

Organism: Contarinia nasturtii

Library:

Name: SM-C1-3rd

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA_oligo_dT

Layout: PAIRED

Runs: 1 run, 32.4M spots, 8.2G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR28707066	32,399,917	8.2G	2.8Gb	2024-04-16

ID:: 32586793

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