Name: LUGEMM14 5-42_p
Instrument: Illumina NovaSeq 6000
Strategy: AMPLICON
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Construction protocol: Samples were collected from GEMMs lung lesions and assessed by MRI quantification of combined lung tumour lesions per animal before & after treatment RNA was extracted using the RNeasy Mini Kit (Qiagen; #74104). RNA concentration was determined by Qubit Flex Fluorometer (Invitrogen), RNA purity was determined using a NanoDrop Eight (Thermo Scientific) and RNA integrity was measured using a 4200 Tapestation (Agilent) Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs; E7760L) or NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs; E7770L) as per manufactuer's guidelines. Ribosomal RNA was removed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs; E7490L) .