Illumina MiSeq paired end sequencing of SAMD00662765 - SRA

Links from BioSample

DRX504258: Illumina MiSeq paired end sequencing of SAMD00662765
1 ILLUMINA (Illumina MiSeq) run: 89,346 spots, 53.8M bases, 30.2Mb downloads

Submitted by: UCPH

Study: Fungal communities associated with seeds of European ash (Fraxinus excelsior)

PRJDB17107 • DRP010984 • All experiments • All runs

show Abstracthide Abstract

Fungal communities associated with seeds of European ash (F. excelsior) were observed using ITS-based metabarcoding. Five to ten seeds were collected from different ramets per genotype and two genotypes were included in this study. The seeds were surfaced sterilized before DNA extraction from stalk and wing using Qiagen DNeasy Plant Pro kit. The primer pair BITS and B58S3 (Bokulich et al., 2013) was used to amplify the ITS1 region before sequencing with Illumina Miseq v3 (2x300 bp).

Sample: ITS1 amplified from genomic DNA from seed wing (no.12 pos.12-15) of Fraxinus excelsior clone 33

SAMD00662765 • DRS356358 • All experiments • All runs

Organism: fungus metagenome

Library:

Name: FexWING_138

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Construction protocol:

Runs: 1 run, 89,346 spots, 53.8M bases, 30.2Mb

Run	# of Spots	# of Bases	Size	Published
DRR520372	89,346	53.8M	30.2Mb	2024-01-05

ID:: 31221154

SRA

Sequence Read Archive

Result Filters

Send to:

Links from BioSample

Supplemental Content

Related information

Recent activity