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SRX22818138: GSM7952034: Run2-ME086_L; Rattus norvegicus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 13.6M spots, 2G bases, 813.8Mb downloads

External Id: GSM7952034_r1
Submitted by: 251 Sir Frederick Banting Driveway, Health Canada
Study: Comparative genomic analysis of Fischer F344 rat livers exposed for 90 days to 3-methylfuran or its parental compound furan.
PRJNA1049840 • SRP476481 • All experiments • All runs
show Abstracthide Abstract
Transcriptomic data obtained by RNA-seq from male Fischer 344 rats treated with furan and 3-methylfuran were compared and contrasted. Microarray data from the same rats treated with furan was also used to compare microarray to RNA seq analysis. Overall design: Compare trascriptomics data between furan and its derivative 3-methylfuran in male Fischer 344 rats using RNA-seq while at the same time comparing microarray data from the same rats treated with furan to that of RNA-seq data from both furan and 3-methylfuran.
Sample: Run2-ME086_L
SAMN38716877 • SRS19799687 • All experiments • All runs
Organism: Rattus norvegicus
Library:
Name: GSM7952034
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from flash frozen tissue samples using Qiagen miRNeasy kits. 20mg of frozen liver was placed in pre-filled tube containing Qiazol reagent and Zirconium beads, then homogenized 2 x for 20 seconds. Subsequent steps were followed as per manufacture's instructions. 3-methylfuran cDNA libraries were constructed using Illumina TruSeq Stranded mRNA protocol. Furan cDNA libraries were prepared with the Illumina Stranded mRNA prep kit (updated release).
Runs: 1 run, 13.6M spots, 2G bases, 813.8Mb
Run# of Spots# of BasesSizePublished
SRR2713618413,614,8242G813.8Mb2024-01-08

ID:
30860083

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