SRA

Eosinophils are well known to regulate host protection from parasites and have been reported to paticipate many other physiologic and pathologic processes. Understanding the role of eosinophils in these processes requires a better understanding of eosinophilopoiesis. Using a zebrafish model, we have identified an eosinophil lineage-specific marker, eslec. Using this marker we have established a Tg(eslec:eGFP) reporter line, which specifically labels zebrafish eosinophil lineage cells from early life through adulthood. Spatial-temporal analysis of eslec+ cells demonstrated organ distribution at the larval stage. By single-cell RNA-Seq of eslec+ cells, tissue-distributed eosinophils were found to have similar differentiation paths but different tissue-specific expression profiles. Genetic analysis demonstrated a Cebp1 and Cebpß transcriptional axis that regulated eosinophilopoiesis, in which Cebp1 directly targeted cebpb to inhibit eosinophil differentiationthe commitment and differentiation of the eosinophil lineage. In summary, this study characterized eosinophil development in multiple dimensions including spatial-temporal patterns, expression profiles, and genetic regulators. The results provide for a better understanding of eosinophilopoiesis. Overall design: For scRNA-Seq analysis of eosinophils in kidney, eosinophils were sorted from adult Tg(eslec:eGFP) kidneys. For scRNA-Seq analysis of renal cells of different genotypes, kidneys were collected from WT, cebp1-/-, and cebpb-/- individuals and prepared into cell suspensions. The cell suspensions were then applied to 10x 3' scRNA-Seq analysis. The scRNA-Seq data of WT, cebp1-/-, and cebpb-/- KM cells were integrated and analyzed together.