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SRX20021912: GSM7188418: Trx_20wk_Mut, rep 1, scRNAseq; Mus musculus; RNA-Seq
4 ILLUMINA (NextSeq 2000) runs: 478M spots, 71.6G bases, 20.7Gb downloads

External Id: GSM7188418_r1
Submitted by: Baylor College of Medicine
Study: SRCAP Mutations Drive Clonal Hematopoiesis Through Epigenetic and DNA Repair Dysregulation [scRNA-Seq]
PRJNA957754 • SRP433550 • All experiments • All runs
show Abstracthide Abstract
To investigate the transcriptomic regulation of Srcap mutant lymphoid-biased expansion after compeititve transplantation Overall design: We established Srcap heterozygous mutant murine competitive transplantation model. Donor test (CD45.2+) HSPCs in the bone marrow were sored and isolated at 20 weeks post-transplantation for scRNA-seq
Sample: Trx_20wk_Mut, rep 1, scRNAseq
SAMN34264336 • SRS17362942 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7188418
Instrument: NextSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: 20,000 donor (CD45.2) HSPCs in the bone marrow were isolated after competitive transplantation for 20 weeks. Each independent Sample were used as input for 10X platform droplet-based library construction. Library was performed using 10x platform 3v31 assay using chromium Single Cell 3' solution without multiplexing according to the manufacter's instructions.
Runs: 4 runs, 478M spots, 71.6G bases, 20.7Gb
Run# of Spots# of BasesSizePublished
SRR2422557363,265,22113.9G4.6Gb2023-10-19
SRR24225574151,219,45621G5.9Gb2023-10-19
SRR24225575137,609,15219.1G5.3Gb2023-10-19
SRR24225576125,932,22317.5G5Gb2023-10-19

ID:
27420437

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