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SRX19994552: GSM7179244: Isolated HSPCs, Doxorubicin IP, 12-hours_1 WT; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 2000) run: 52.2M spots, 5.3G bases, 1.5Gb downloads

External Id: GSM7179244_r1
Submitted by: Baylor College of Medicine
Study: SRCAP Mutations Drive Clonal Hematopoiesis Through Epigenetic and DNA Repair Dysregulation [RNA-seq]
PRJNA956488 • SRP433062 • All experiments • All runs
show Abstracthide Abstract
To investigate how Srcap mutations confer mutant hematopoietic stem and progenitor cells (HSPCs) with selective advantage after exposure to cellular stressors, transplantation and doxorubicin treatment We established Srcap heterozygous mutant murine model and utilized competitive bone marrow transplantation to assess the transcriptome of donor cells using RNA-seq Overall design: Transcriptomic profiling of RNA-seq data for isolated donor test (CD45.2+) cells 20 weeks after competitive bone marrow transplantation
Sample: Isolated HSPCs, Doxorubicin IP, 12-hours_1 WT
SAMN34224115 • SRS17339608 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7179244
Instrument: NextSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was harvested from 30,000 sorted HSPCs in the bone marrow and isolated using Allprep DNA/RNA Micro Kit (Qiagen) of each independent Sample for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using True-Seq Stranded mRNA library preparation kit (Illumina, 20020594) based on manufacturer's protocol.
Runs: 1 run, 52.2M spots, 5.3G bases, 1.5Gb
Run# of Spots# of BasesSizePublished
SRR2419780752,225,6035.3G1.5Gb2023-10-19

ID:
27385873

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