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SRX19989012: AMPLICON of Severe acute respiratory syndrome coronavirus 2: Human E3335432-ERLA293
1 ILLUMINA (Illumina NovaSeq 6000) run: 1.3M spots, 279.8M bases, 12.4Mb downloads

External Id: E3335432-ERLA293-2-8100638-SRA
Design: The E3335432-ERLA293 library was prepared using the Molecular Loop Viral RNA Target Capture Kits (Molecular Loop) in accordance with manufacturer's recommendations. Briefly, 6uL of RNA was reverse transcribed and capture probes annealed in a 16 hour incubation at 55ºC. The probes were then enzymatically circularized to capture the viral genome and add Unique Molecular Indexes followed by amplification of circularized cDNA targets using 27 cycles of PCR. Libraries were pooled by volume and cleaned-up by magnetic bead purification. Final libraries were quantified using fluorescent-based assays including PicoGreen (Life Technologies) or Qubit Fluorometer (Invitrogen), and Fragment Analyzer (Advanced Analytics) and sequenced on Illumina NovaSeq 6000 with 2x150bp reads.
Submitted by: NYGC
Study: New York Genome Center (NYGC) SARS-CoV-2 Genome sequencing and assembly
PRJNA766678 • SRP377438 • All experiments • All runs
show Abstracthide Abstract
New York Genome Center (NYGC) Whole genome sequencing of SARS-CoV-2 as part fo the COVID-19 Genomics Research Network viral surveillance effort.
Sample: SARS-CoV-2: Clinical Severe acute respiratory syndrome coronavirus 2 sample collected from NYC metropolitan area
SAMN34221158 • SRS17334180 • All experiments • All runs
Organism: Severe acute respiratory syndrome coronavirus 2
Library:
Name: E3335432-ERLA293-2-8100638
Instrument: Illumina NovaSeq 6000
Strategy: AMPLICON
Source: VIRAL RNA
Selection: PCR
Layout: PAIRED
Runs: 1 run, 1.3M spots, 279.8M bases, 12.4Mb
Run# of Spots# of BasesSizePublished
SRR241918401,345,896279.8M12.4Mb2023-04-17

ID:
27380333

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