Sequencing of RNA from C. fleckeri tentacle - SRA

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SRX891607: Sequencing of RNA from C. fleckeri tentacle
1 ILLUMINA (Illumina HiSeq 2000) run: 46.7M spots, 9.4G bases, 5.8Gb downloads

Design: Tentacles were excised and immediately preserved in RNAlater (Life Technologies). Total RNA was isolated from the C. fleckeri tentacles of a single specimen using a handheld rotor-stator homogenizer (QIAgen) and TRIzol (Life Technologies), according to the manufacturer’s instructions. The RNA was DNase-treated, purified using a Nucleospin RNA II kit (Macherey-Nagel) and eluted in nuclease-free water. RNA purity, concentration and integrity were evaluated using a NanoDrop 2000 UV-Vis spectrophotometer and an Agilent 2100 Bioanalyzer with a RNA 6000 Pico kit. Purified RNA (5 μg; RIN 9.1) was submitted to the Ramaciotti Centre for Genomics (Sydney, Australia) for RNA-seq library construction and 100 bp paired-end sequencing on an Illumina HiSeq2000 sequencer.

Submitted by: QIMR Berghofer MRI

Study: Chironex fleckeri Tentacle Transcriptome

PRJNA276493 • SRP055535 • All experiments • All runs

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Transcriptome of C. fleckeri (box jellyfish) tentacle

Sample: Tentacle sample from Chironex fleckeri

SAMN03375745 • SRS857947 • All experiments • All runs

Organism: Chironex fleckeri

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC