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SRX883398: GSM1613594: C1 Fixed cell 9; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 2.8M spots, 205.3M bases, 92.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Single mammalian cells compensate for differences in cellular volume and DNA copy number through independent global transcriptional mechanisms
PRJNA275801 • SRP055153 • All experiments • All runs
show Abstracthide Abstract
We performed single-cell and bulk transcriptome profiling in two different human cell lines. We performed single-cell RNA sequencing in live and fixed cells. Overall design: Single cell RNA sequencing of live and fixed cells, bulk RNA sequencing in two cell lines.
Sample: C1 Fixed cell 9
SAMN03353393 • SRS850693 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Fixed cells were added to the Fluidigm C1 Single Cell Auto Prep System at 200K cells/ml. ERCC control mix 1 was added at a dilution of 1:10,000. First- and second-strand synthesis were performed on the C1 Single Cell Auto Prep System. Final library prep was performed using the Illumina Nextera XT DNA sample preparation kit, using the modified protocol in the C1 manual.
Experiment attributes:
GEO Accession: GSM1613594
Links:
External link:
Runs: 1 run, 2.8M spots, 205.3M bases, 92.1Mb
Run# of Spots# of BasesSizePublished
SRR18106702,765,379205.3M92.1Mb2015-03-05

ID:
1266249

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