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SRX19461715: Fungal ITS amplicon sequencing of root metagenome: sample run2-299
1 ILLUMINA (Illumina MiSeq) run: 75,021 spots, 43.9M bases, 27.7Mb downloads

Design: Fungal ITS amplicon library was created using a barcoded fusion primer PCR protocol using Pfx50 polymerase (Thermo Fisher Scientific), size selected, and sequenced on the Illumina MiSeq platform. Sequence data was minimally processed, sequences were exported directly from the sequencing platform and only the barcode (index sequence) was trimmed prior to SRA submission. SRA submission generated with AMPtk 1.5.0
Submitted by: USDA Forest Service, Northern Research Station
Study: Root-associated fungal communities are influenced more by soils than by plant-host root traits in a Chinese tropical forest
show Abstracthide Abstract
Forest fungal communities are shaped by the interactions between host tree root systems and the associated soil conditions. We investigated how the soil environment, root morphological traits, and root chemistry influence root-inhabiting fungal communities in three tropical forest sites of varying successional status in Xishuangbanna, China.For 150 trees of 66 species, we measured root morphology and tissue chemistry. Tree species identity was confirmed by sequencing rbcL and root-associated fungal (RAF) communities were determined using high-throughput ITS2 sequencing.
Sample: XTBG_299_BNFZH01ABC_01_rainforest 1-HA at flux tower
SAMN33392127 • SRS16854341 • All experiments • All runs
Organism: root metagenome
Library:
Name: run2-299
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: GENOMIC
Selection: RANDOM PCR
Layout: PAIRED
Runs: 1 run, 75,021 spots, 43.9M bases, 27.7Mb
Run# of Spots# of BasesSizePublished
SRR2357484475,02143.9M27.7Mb2023-02-21

ID:
26736423

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