genome sequence of NGB-FR 75 - SRA

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SRX19128555: genome sequence of NGB-FR 75
1 ILLUMINA (Illumina MiSeq) run: 1.1M spots, 534.1M bases, 294.1Mb downloads

Design: Bacterial DNA was extracted from freshly sub-cultured colonies using the QIAamp DNA Mini Kit (Qiagen, cat # 51304) according to the manufacturers instructions, and the DNA concentration was measured using a Denovix Fluorometer (Denovix, USA). The genomic DNA was stored at -20C. Total of bacterial DNA (1 ng) was used in the library preparation. The library was prepared with Nextera XT DNA Library Preparation Kit (FC-131-1096, Illumina, USA), according to the manufacturers instructions. In brief, transposons were used to fragment the DNA, subsequently adapter sequences were added onto the DNA template, the product was then size-selected for optimum insert length, enriched and quantified. Sequencing was carried out with the MiSeq reagent kit 600 v3 (Illumina, USA) on the Illumina MiSeq, generating, on average 301 basepair paired-end reads.

Submitted by: msa univeristy

Study: Klebsiella, Enterobacter Genome sequencing and assembly

PRJNA923792 • SRP418571 • All experiments • All runs

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The aim of this project is to sequence nodule-associated bacteria (NAB) isolates corresponding to the family Enterobacteriaceae (the genera Klebsiella, Enterobacter) which are isolated from the root nodules of faba bean grown under various soil conditions in Egypt. The potential effect of these strains as plant growth-promoting bacteria was previously confirmed in-vitro and in-vivo. The project aim ia to identify the genetic machinery of these enterobacteria to improve plant fitness and development and shed light on their inactive influence on pathogenicity.

Sample: genome sequence of NGB-FR 75

SAMN32759073 • SRS16540963 • All experiments • All runs

Organism: Klebsiella variicola

Library:

Name: Klebsiella variicola FR 75

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 1.1M spots, 534.1M bases, 294.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR23178144	1,093,185	534.1M	294.1Mb	2023-01-21

ID:: 26318573

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