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SRX17142382: 16S rRNA sequencing of Philophthalmus attenuatus: Pen_ColT77soldier
1 ILLUMINA (Illumina MiSeq) run: 85,452 spots, 42.9M bases, 26.7Mb downloads

Design: libraries pools were prepared for sequencing targeting the V4 hypervariable region of the prokaryotic bacterial 16S SSU rRNA gene. DNA extraction, and library preparations were performed as described in Jorge et al. (2020; doi: 10.3389/fmicb.2020.00954), including the use of the two types of ZymoBIOMICS microbial community standards samples (MCS and MCS DNA).
Submitted by: University of Otago
Study: Bacterial community dynamics following antibiotic exposure in a trematode parasite.
show Abstracthide Abstract
Parasites harbour rich microbial communities that may play a role in host-parasite interactions, from influencing the parasites infectivity to modulating its virulence. Experimental manipulation of a parasites microbes would be essential, however, in order to establish their causal role. Here, we tested whether indirect exposure of a trematode parasite within its snail intermediate host to a variety of antibiotics could alter its bacterial community. Based on sequencing the prokaryotic 16S SSU rRNA gene, we characterised and compared the bacterial community of the trematode Philophthalmus attenuatus before, shortly after, and weeks after exposure to different antibiotics, i.e penicillin, colistin, gentamicin, with distinct activity spectra.
Sample:
SAMN30360445 • SRS14715083 • All experiments • All runs
Library:
Name: 5965-071-20-1
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 85,452 spots, 42.9M bases, 26.7Mb
Run# of Spots# of BasesSizePublished
SRR2113015885,45242.9M26.7Mb2022-08-19

ID:
23944350

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