Hyb-seq of Cardamine uliginosa seiGRJS1 - SRA

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SRX14997503: Hyb-seq of Cardamine uliginosa seiGRJS1
1 ILLUMINA (Illumina MiSeq) run: 785,974 spots, 255.4M bases, 127Mb downloads

Design: Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the recommended manufacturers protocol. Genomic DNA (400 ng per accession) was fragmented with Covaris M220 sonicator (Woburn, MA, USA) to a target fragment size of 500 bp. Adaptor-ligated DNA fragments were purified with the QIAquick PCR Purification Kit (Qiagen) and size-selected using SPRIselect beads (Beckman Coulter, MA, USA) to 500600 bp size range. PCR enrichment with eight cycles was performed using index primers from NEBNext Multiplex Oligos for Illumina. The amplified libraries were cleaned up with AMPure XP beads (Beckman Coulter), measured with Qubit 2.0 fluorometer (ThermoFisher Scientific), and pooled equimolarly (24 accessions/pool). The pooled libraries were size-selected using SPRIselect beads as above and measured again with Qubit 2.0. Aliquots containing 200-400 ng were enriched by hybridization with synthesized RNA baits (26 h at 65C) using the MYbaits kit, following the protocol v. 3.02 (Arbor Biosciences). The target-enriched libraries were amplified by PCR with nine cycles using the KAPA HiFi HotStart mix (Kapa Biosystems, Cape Town, South Africa) and purified with the QIAquick PCR Purification Kit. Enriched and unenriched library aliquots were pooled in a ratio 2:1, finally purified with AMPure XP beads, and submitted for sequencing with 150 bp paired end reads on an Illumina MiSeq system.

Submitted by: Plant Science and Biodiversity Centre; Slovak Academy of Sciences

Study: Cardamine perennials in SW Asia Raw sequence reads

PRJNA830631 • SRP372240 • All experiments • All runs

show Abstracthide Abstract

Phylogenomics of hygrophytic Cardamine perennials in SW Asia: the evolutionary history characterized by recent speciation, long-term range stability, and rare allopolyploidy.

Sample: Plant sample from Cardamine uliginosa

SAMN27735400 • SRS12746732 • All experiments • All runs

Organism: Cardamine uliginosa

Library:

Name: seiGRJS1

Instrument: Illumina MiSeq

Strategy: Targeted-Capture

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 785,974 spots, 255.4M bases, 127Mb

Run	# of Spots	# of Bases	Size	Published
SRR18919650	785,974	255.4M	127Mb	2023-02-21

ID:: 21452234

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