RNA-Seq of Solanum pennellii: leaf - SRA

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SRX14706163: RNA-Seq of Solanum pennellii: leaf
1 ILLUMINA (Illumina NovaSeq 6000) run: 32.1M spots, 9.6G bases, 2.8Gb downloads

Design: Total RNA was extracted from leaves of control and VIGS plants of S. pennellii using the RNAqueous Total RNA Isolation kit (ThermoFischer), followed by treatment with the TURBO DNA-free kit (ThermoFischer) to remove genomic DNA. RNA-Seq libraries were prepared from poly(A)-selected RNA samples using the NEXTFLEX Poly(A) Beads 2.0 and Rapid Directional RNA-Seq kit 2.0 (PerkinElmer), and then sequenced on the Illumina NovaSeq 6000 SP v1.5 (2x150) platform at the Texas A&M Genomics and Bioinformatics service center. Sequence cluster identification, quality prefiltering, base calling and uncertainty assessment were done in real time using Illuminas NCS 1.0.2 and RFV 1.0.2 software with default parameter settings. Sequencer .cbcl basecall files were demultiplexed and formatted into .fastq files using bcl2fastq 2 2.19.0 script configureBclToFastq.pl.

Submitted by: Texas A&M University

Study: virus-induced gene silencing of ASTF1

PRJNA821975 • SRP367098 • All experiments • All runs

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ASTF1 is an AP2/ERF-family transcription factor, which is required for acylsugar biosynthesis. Virus-induced gene silencing was used for functional validation of ASTF1.

Sample:

SAMN27162250 • SRS12468967 • All experiments • All runs

Organism: Solanum pennellii

Library:

Name: 21323Mck_ASTF1_S7_L001

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 32.1M spots, 9.6G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR18577196	32,063,998	9.6G	2.8Gb	2023-04-01

ID:: 21043231

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