show Abstracthide AbstractA principal vector of malaria in the Americas, Anopheles albimanus, exhibits diverse geographical distribution and phenotypic variation. Hence, a high-quality reference genome from geographically distant populations is essential is to understand and capture genetic variation in this species. In this study, we sequenced and assembled the whole genome sequences of two reference strains of An. albimanus, originating from El Salvador (STECLA) and Colombia (Cartagena, CART), using a combination of long-read PacBio and short-read Illumina-HiSeq technologies.