Genome sequencing of Fomes fomentarius collected at the Massane Forest - SRA

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SRX13416930: Genome sequencing of Fomes fomentarius collected at the Massane Forest
1 ILLUMINA (Illumina HiSeq 2000) run: 4.8M spots, 1.4G bases, 580.5Mb downloads

Design: The genomic DNA is randomly fragmented to a size of 350bp by shearing, then DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system) and resulted libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR. Paired-end sequencing was performed using Illumina (HiSeq 2000) at a coverage of 20X and a read length of 150bp for each sample.

Submitted by: University of Perpignan Via Domitia

Study: Horizontal transfer characterization in the Massane Forest

PRJNA788424 • SRP350496 • All experiments • All runs

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This project aims to characterize and study the horizontal transfer between non-model species in a natural ecosystem: the Massane forest located in southern France.

Sample:

SAMN24102160 • SRS11318561 • All experiments • All runs

Organism: Fomes fomentarius

Library:

Name: FF_12j

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 4.8M spots, 1.4G bases, 580.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR17237821	4,770,606	1.4G	580.5Mb	2021-12-15

ID:: 18550312

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