whole genome sequencing of Ophidiomyces ophidiicola strains - SRA

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SRX13394482: whole genome sequencing of Ophidiomyces ophidiicola strains
1 ILLUMINA (Illumina HiSeq 2500) run: 2.4M spots, 608.9M bases, 221.9Mb downloads

Design: Strains were grown in 20 mL of Sabouraud dextrose broth at 24C for 9-15 days. Fungal biomass from each culture was flash-frozen in liquid nitrogen, lyophilized, pulverized using a pestle and glass beads, resuspended in 700 L of LETS buffer (20 mM EDTA [pH 8.0], 0.5% sodium dodecyl sulfate, 10mM Tris-HCl [pH 8.0], 0.1 M LiCl), and DNA was extracted using the phenol-chloroform method. Library preparation (Nextera XT DNA Library Prep Kit) and whole genome sequencing was performed by the University of Wisconsin-Madison Biotechnology Center (Madison, Wisconsin) on an Illumina HiSeq system (2 x 125 bp read lengths).

Submitted by: U.S. Geological Survey - National Wildlife Health Center

Study: Ophidiomyces ophiodiicola genome sequencing and assembly

PRJNA780910 • SRP350492 • All experiments • All runs

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Ophidiomyces ophidiicola whole genome sequencing

Sample:

SAMN23192515 • SRS11297658 • All experiments • All runs

Organism: Ophidiomyces ophidiicola

Library:

Name: NWHC_44736-90

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 2.4M spots, 608.9M bases, 221.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR17214498	2,416,227	608.9M	221.9Mb	2022-04-28

ID:: 18479216

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