Listeria monocytogenes Strain J2-1091 - SRA

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SRX320103: Listeria monocytogenes Strain J2-1091
9 PACBIO_SMRT (PacBio RS) runs: 735,669 spots, 1.8G bases, 6.6Gb downloads

Submitted by: UCD-100K

Study: Major food bacterial pathogens in the united states and around the world, including Salmonella enterica, E. coli, Shigella, Listeria monocytogenes, Campylobacter Jejuni, and Vibrio parahaemolyticus genome sequencing

PRJNA203445 • SRP022914 • All experiments • All runs

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University of California at Davis 100K Pathogen Genome Project. Major bacterial pathogens in the United States and around the world, including Salmonella enterica, E. coli, Shigella, Listeria monocytogenes, Campylobacter Jejuni, Vibrio parahaemolyticus. This project also contains WGS from many food-associated organisms that have unique traits.

Sample: Pathogen sample from Listeria monocytogenes

SAMN02203123 • SRS456498 • All experiments • All runs

Organism: Listeria monocytogenes

Library:

Instrument: PacBio RS

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: SMRTbell sequencing libraries were generated using standard procedures (version C2; http://www.pacb.com/samplenet/PC_10kb_Template_Preparation_and_Sequencing.pdf). gDNA was sheared to an average size of approximately 10 kb using g-TUBEs (Covaris). Fragmented DNA underwent DNA Damage Repair, End Repair, and blunt hairpin adapters were ligated onto each end. Incompletely formed SMRTbell templates were degraded with a combination of Exo III and Exo VII. SMRTbell templates were magbead loaded and sequenced with C2 chemistry.

Spot descriptor:

1 forward

Runs: 9 runs, 735,669 spots, 1.8G bases, 6.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR932082	81,741	186.7M	714.7Mb	2013-07-12
SRR932083	81,741	183.4M	694Mb	2013-07-12
SRR932084	81,741	216M	807.6Mb	2013-07-12
SRR932085	81,741	217.3M	812.3Mb	2013-07-12
SRR932086	81,741	183.8M	718.6Mb	2013-07-12
SRR932087	81,741	194.6M	748.7Mb	2013-07-12
SRR932088	81,741	175.8M	677.8Mb	2013-07-12
SRR932089	81,741	216.4M	824.1Mb	2013-07-12
SRR932090	81,741	204.7M	781.9Mb	2013-07-12

ID:: 447172

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