Whole genome sequencing of Bacteroides fragilis group isolates - SRA

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SRX11588770: Whole genome sequencing of Bacteroides fragilis group isolates
1 ILLUMINA (NextSeq 500) run: 2.1M spots, 625.4M bases, 233.7Mb downloads

Design: gDNA was extracted using the the QIAamp/MoBio BiOstic Bacteremia DNA Kit. 0.5 ng gDNA was used to prepare Illumina sequencing libraries using the Nextera kit (Illumina, San Diego, CA, USA). All libraries were pooled at equal concentrations and sequenced at a target depth of 2 million reads per sample.

Submitted by: Washington University School of Medicine in St. Louis

Study: Bacteroides fragilis group isolates from human infections

PRJNA745162 • SRP330269 • All experiments • All runs

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This study includes genomes from 194 Bacteroides fragilis group isolates, including 174 isolates collected at the Barnes-Jewish Hospital in St. Louis, MO from 2018-2020, as well as 20 banked B. fragilis sensu stricto isolates enriched for carbapenem resistance. Genome sequences are used for phylogenomic studies to validate the accuracy of species identification tools commonly used in clinical settings. Furthermore, these genomes are used to identify species-specific genetic elements related to antibiotic resistance for comparison with phenotypic antibiotic resistance.

Sample:

SAMN20148166 • SRS9623275 • All experiments • All runs

Organism: Bacteroides thetaiotaomicron

Library:

Name: BFG_SL_BJH_202

Instrument: NextSeq 500

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 2.1M spots, 625.4M bases, 233.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR15283693	2,084,747	625.4M	233.7Mb	2021-12-17

ID:: 15501539

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