Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was harvested from cell pellets by bead-beating (5 min, 30 sec on and off cooling on ice) followed by RNA extraction using the Qiagen RNEasy Mini Kit protocol. The samples were prepared using the Illumina TruSeq RNA library prep kit, starting with isolated mRNA since the organism was yeast. Approximately 1 ug of total RNA was used and polyA mRNA selected using the Illumina TruSeq RNA library prep kit. After multiplexing, the samples were pooled and sequenced on a HiSeq2500 in one lane, single read, 50 cycles.