SRX9689851: ATCC-700206
1 ILLUMINA (NextSeq 500) run: 1.8M spots, 542.2M bases, 227.9Mb downloads
Design: Isolates were grown on Columbia 5% Sheep Blood Agar (bioMrieux, Marcy-ltoile, France) and DNA was extracted using the QIACube with the QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany). After quality control of the DNA by Tapestation (Agilent, Santa Clara, USA), tagmentation libraries were generated as described by the manufacturer (Nextera Flex kit, Illumina, San Diego, USA). The genomes were sequenced under 24x multiplexing using a paired end 150 base pairs V3 reaction kit on an Illumina NextSeq500 instrument reaching an average coverage of approximately 60-fold for all isolates.
Submitted by: University of Basel
Study:
Diverse set of strains from clinically relevant bacterial species, posing a challenge for identification by MALDI-TOF MSshow Abstracthide AbstractIn this study we aim to define MALDI-TOF mass spectral quality and identify simple protocols which could increase spectral quality in a routine workflow
Library:
Name: 31
Instrument: NextSeq 500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 1.8M spots, 542.2M bases, 227.9Mb