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SRX5110628: Microfluidic amplification and MiSeq paired-end sequencing of Lachemilla fulvescens 2012_408
1 ILLUMINA (Illumina MiSeq) run: 51,241 spots, 28.6M bases, 18.5Mb downloads

Design: Microfluidic PCRs were carried out either in an Access Array or Juno system (Fluidigm, San Francisco, California, USA) following the manufacturers protocols. To remove unused reagents and/or undetected primer dimers smaller than 350 bp, each pool was purified with 0.6 AMPure XP beads (AgencourtT, Beverly, Massachusetts, USA). PCR pools were analyzed in a Bioanalyzer High-Sensitivity Chip (Agilent Technologies, Santa Clara, California, USA), and standardized to 13 pM using the KAPA qPCR kit (KK4835; Kapa Biosystems, Woburn, Massachusetts, USA) on an ABI StepOnePlus Real-Time PCR System (Life Technologies, Grand Island, New York, USA). The resulting pools were multiplexed and sequenced in an Illumina MiSeq (San Diego, California, USA) with 300 bp paired-end reads.
Submitted by: University of Minnesota
Study: Microfluidic PCR target enrichment of the genus Lachemilla (Rosaceae)
PRJNA508567 • SRP172980 • All experiments • All runs
show Abstracthide Abstract
This study focuses in detecting polyploidy in the genus Lachemilla (Rosaceae) using a phylogenetic approach. We amplify and sequence the nuclear ribosomal cistron (ETS, ITS1 and ITS2) as wells as 48 chloroplast regions using microfluidic PCR and Illumina sequencing.
Sample: Lachemilla_fulvescens_2012_408
SAMN10531673 • SRS4119811 • All experiments • All runs
Organism: Alchemilla fulvescens
Library:
Name: 2012_408
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 51,241 spots, 28.6M bases, 18.5Mb
Run# of Spots# of BasesSizePublished
SRR829600251,24128.6M18.5Mb2018-12-10

ID:
6924070

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