Microfluidic amplification and MiSeq paired-end sequencing of Lachemi... - SRA

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SRX5110630: Microfluidic amplification and MiSeq paired-end sequencing of Lachemilla fulvescens 2012_368
1 ILLUMINA (Illumina MiSeq) run: 13,068 spots, 7.3M bases, 4.1Mb downloads

Design: Microfluidic PCRs were carried out either in an Access Array or Juno system (Fluidigm, San Francisco, California, USA) following the manufacturers protocols. To remove unused reagents and/or undetected primer dimers smaller than 350 bp, each pool was purified with 0.6 AMPure XP beads (AgencourtT, Beverly, Massachusetts, USA). PCR pools were analyzed in a Bioanalyzer High-Sensitivity Chip (Agilent Technologies, Santa Clara, California, USA), and standardized to 13 pM using the KAPA qPCR kit (KK4835; Kapa Biosystems, Woburn, Massachusetts, USA) on an ABI StepOnePlus Real-Time PCR System (Life Technologies, Grand Island, New York, USA). The resulting pools were multiplexed and sequenced in an Illumina MiSeq (San Diego, California, USA) with 300 bp paired-end reads.

Submitted by: University of Minnesota

Study: Microfluidic PCR target enrichment of the genus Lachemilla (Rosaceae)

PRJNA508567 • SRP172980 • All experiments • All runs

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This study focuses in detecting polyploidy in the genus Lachemilla (Rosaceae) using a phylogenetic approach. We amplify and sequence the nuclear ribosomal cistron (ETS, ITS1 and ITS2) as wells as 48 chloroplast regions using microfluidic PCR and Illumina sequencing.

Sample: Lachemilla_fulvescens_2012_368

SAMN10531671 • SRS4119813 • All experiments • All runs

Organism: Alchemilla fulvescens

Library:

Name: 2012_368

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 13,068 spots, 7.3M bases, 4.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR8296000	13,068	7.3M	4.1Mb	2018-12-10

ID:: 6924072

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