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SRX5058220: GSM3487586: TGFB1_spheroids_1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 37.8M spots, 7.6G bases, 3Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptional profile of stemness in the ovarian surface epithelium
PRJNA506731 • SRP170547 • All experiments • All runs
show Abstracthide Abstract
We leverage RNA sequencing to identify the transcriptional changes assocaited with several experimental conditions that induce a stemness phenotype in the ovarian surface epithelium, including spheroid culture, Snail overexpression, and BRCA1 deletion Overall design: RNA sequencing of mOSE cultured as monolayers or spheroids (+/- TGFB1), and monolayer cells overexpressing Snail, or following deletion of BRCA1 (n=3 for each experiment)
Sample: TGFB1_spheroids_1
SAMN10473197 • SRS4074389 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was collected using the RNeasy Kit (Qiagen) Libraries were generated from 250 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs). The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs. Libraries were quantified using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument.
Experiment attributes:
GEO Accession: GSM3487586
Links:
Runs: 1 run, 37.8M spots, 7.6G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR823995637,845,6707.6G3Gb2020-06-01

ID:
6826837

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