RRBS of Octopus bimaculoides: 30 dpf whole-body hatchling - SRA

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SRX13138005: RRBS of Octopus bimaculoides: 30 dpf whole-body hatchling
1 ILLUMINA (NextSeq 550) run: 28.9M spots, 8.5G bases, 3.6Gb downloads

Design: DNA quantity and quality was assesed by Qbit and Bioanalyer. 1000ng of DNA was used for library prep. Libraries were prepared according to Garrett-Bakelman et al., 2015 with some modifications. The adaptors used for multiplexing were purchased separately (Next Multiplex Methylated Adaptors - New England Biolabs). Libraries were size-selected by dual-step purification with Ampure XP Magnetic Beads (Beckman Coulter, Agencourt) to specifically select a region of fragments from 175 bp to 670 bp. Bisulfite conversion was performed with Lightning Methylation Kit (ZYMO Research) by following the manufacturers instructions. Libraries were amplified using KAPA HiFi HotStart Uracil+ Taq polymerase (Roche) and purified with Ampure XP Magnetic Beads (Beckman Coulter, Agencourt) before sequencing.

Submitted by: New York University Abu Dhabi

Study: DNA methylation landscape of 30 day old Octopus bimaculoides hatchlings (whole body)

PRJNA780401 • SRP346087 • All experiments • All runs

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In order to determine the distribution of 5-methyl cytosine throughout the genome of a juvenile Octopus bimaculoides, we carried out reduced representation bisulfite sequencing on a 30 day old hatchling.

Sample: RRBS_WT_30 dpf Octopus Hatchlings

SAMN23139394 • SRS11071204 • All experiments • All runs

Organism: Octopus bimaculoides

Library:

Name: DOJ1

Instrument: NextSeq 550

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: Reduced Representation

Layout: PAIRED

Runs: 1 run, 28.9M spots, 8.5G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR16946064	28,863,110	8.5G	3.6Gb	2023-01-31

ID:: 17922919

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