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SRX8621731: Aspergillus flavus from Ethiopia peanut
1 ILLUMINA (Illumina HiSeq 4000) run: 15.2M spots, 4.6G bases, 1.7Gb downloads

Design: Barcode-indexed sequencing libraries were generated from genomic DNA samples sheared on an E220 Focused Ultrasonicator (Covaris, Woburn, MA). The sheared DNA samples were size-selected with a double-sided SPRI-protocol with Ampure XP beads (Beckman Coulter, Brea, CA) using bead-to-sample ratios of 0.6x and 0.73x. The size-selected DNA samples were converted to sequencing libraries using a Kapa Hyper Library Preparation Kit (Kapa Biosystems-Roche, Basel, Switzerland). The libraries were amplified with six PCR cycles and analyzed with a Bioanalyzer 2100 instrument (Agilent, Santa Clara, CA), quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA), and combined in a pool at equimolar ratios.
Submitted by: USDA
Study: Aspergillus flavus E1445 Genome sequencing and assembly
PRJNA523235 • SRP268905 • All experiments • All runs
show Abstracthide Abstract
Isolate from peanut from Ethiopia
Sample: Aspergillus flavus from Ethiopia peanut, E1445
SAMN10967342 • SRS6910472 • All experiments • All runs
Organism: Aspergillus flavus
Library:
Name: E1445
Instrument: Illumina HiSeq 4000
Strategy: WGS
Source: GENOMIC
Selection: RT-PCR
Layout: PAIRED
Runs: 1 run, 15.2M spots, 4.6G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR1209590415,212,1474.6G1.7Gb2020-06-26

ID:
11205851

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