show Abstracthide AbstractA comprehensive investigation of the phylogenetic composition and metabolic potential of Sungai Klah, which is located in Perak, Malaysia. It is the second hottest geothermal springs in Malaysia. The hotspring is a streamer-like and several spring heads with boiling temperature (100-110 °C) are presence. Other parts of the streaming water exhibit the temperature ranging from 50-100 °C. Little knowledge on the types of hyper- and thermophilic within this hot spring is known. Furthermore, this high temperature environment condition serves as a source for understanding the genetic diversity, population structure, and ecological roles of these boiling point-tolerant microorganisms.