GSM1659655: PXO99A with dTal280 replicate 1; Oryza sativa Japonica Gr... - SRA

SRX997482: GSM1659655: PXO99A with dTal280 replicate 1; Oryza sativa Japonica Group; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 29.6M spots, 3G bases, 2.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Host transcriptional reprogramming in response to the rice bacterial blight pathogen Xanthomonas oryzae pv.oryzae constitutively expressing the X. oryzae pv. oryzicola TAL2a effector from the high copy vector pKEB31

PRJNA281369 • SRP057260 • All experiments • All runs

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We performed RNA-Seq of leaves of Oryza sativa L. ssp. japonica cv. Nipponbare 48 hours after inoculation with Xanthomonas oryzae pv. oryzae strain PXO99A heterologously expressing the Tal2a effector, the designer TAL effector dT280 which targets a sequence overlapping the predicted Tal2a binding sequence in UCH, or the Tal11b effector. Results provide insight into the genes differentially regulated in a Tal2a- and dT280-specific manner. Overall design: Examination of mRNA levels in Oryza sativa L. ssp. japonica cv. Nipponbare leaves at 48 hours after inoculation. Each leaf was considered a separate biological replicate.

Sample: PXO99A with dTal280 replicate 1

SAMN03487371 • SRS911699 • All experiments • All runs

Organism: Oryza sativa Japonica Group

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted with the Qiagen RNeasy kit and manufacturer's protocol. These libraries were prepped with the Illumina TruSeq RNA Sample Prep v2 kit

Experiment attributes:

GEO Accession: GSM1659655

Links:

External link: GEO Sample GSM1659655

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 29.6M spots, 3G bases, 2.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR1976514	29,595,813	3G	2.1Gb	2015-05-01

ID:: 1448368

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