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SRX9708459: CENP-A ChIP-Seq of CHM13hTERT
1 ILLUMINA (NextSeq 500) run: 65.7M spots, 19.8G bases, 5.9Gb downloads

Design: CHM13hTERT chromatin was digested with MNase, and CENP-A-containing nucleosomes were immunoprecipitated using the ADI-KAM-CC006-E (Enzo) antibody. Libraries were prepared and sequenced via paired-end, 150x150bp sequencing on the NextSeq500.
Submitted by: Telomere-to-Telomere (UW subgroup) (T2T-UWsubgroup)
Study: T2T - CHM13
show Abstracthide Abstract
We have sequenced the CHM13hTERT human cell line on the Oxford Nanopore GridION. We have also sequenced approximately 50x coverage using 10X Genomics as well as BioNano DLS and Arima Genomics HiC. PacBio data for this cell line has been previously generated by the Washington University School of Medicine and the University of Washington, and is available from NCBI SRA.Human genomic DNA was extracted from the cultured cell line. As the DNA is native, modified bases will be preserved. We followed Josh Quick's ultra-long read (UL) protocol for library preparation and sequencing.
Sample: Human sample from CHM13htert cell line from Homo sapiens
SAMN03255769 • SRS798661 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: CENP-A_ChIP_2
Instrument: NextSeq 500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Runs: 1 run, 65.7M spots, 19.8G bases, 5.9Gb
Run# of Spots# of BasesSizePublished
SRR1327868365,658,01819.8G5.9Gb2021-02-17

ID:
12697366

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