ZW_30d_biological replicate 3 - SRA

SRX7906445: ZW_30d_biological replicate 3
1 ILLUMINA (Illumina HiSeq 2500) run: 33.7M spots, 10.1G bases, 3Gb downloads

Design: Total RNA was extracted from gonads of ZZ and ZW fishes using the Trizol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturers instruction, and purified using DNaseI to eliminate genomic DNA contamination. RNA degradation and contamination were monitored on 1% agarose gels and a Nanodrop spectrophotometer. Illumina sequencing was carried out at Novogene Bioinformatics Technology Co., Ltd., in Beijing, China. Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), according to the manufacturers protocol. Index codes were added to attribute sequences to each sample. The prepared libraries were sequenced on an Illumina Hiseq 2500 platform, and 150bp paired-end reads were generated.

Submitted by: University of Vienna

Study: Oreochromis niloticus and O. aureus Genome sequencing and assembly

PRJNA609616 • SRP251337 • All experiments • All runs

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We sequenced two tilapia genomes, Nile tilapia (Oreochromis niloticus) and blue tilapia (O. aureus), using Nanopore long-read sequencing. We further used Hi-C to link the scaffolds into chromosomes.

Sample: ZW_30d_biological replicate 3

SAMN14363947 • SRS6314051 • All experiments • All runs

Organism: Oreochromis aureus

Library:

Name: ZW_30d_3

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 33.7M spots, 10.1G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR11301104	33,749,154	10.1G	3Gb	2020-07-15

ID:: 10341334

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