GSM1440949: BarcodedLibrary19_G_66h_DI; Anopheles coluzzii; RNA-Seq - SRA

SRX658351: GSM1440949: BarcodedLibrary19_G_66h_DI; Anopheles coluzzii; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 10.6M spots, 1.1G bases, 780.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

PRJNA255780 • SRP044683 • All experiments • All runs

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We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus. Overall design: After rearing in freshwater, both young and old larval instars of each species were briefly (6 h) exposed to either saltwater (SW) or freshwater (FW) conditions to test the impact of salinity on mRNA levels.

Sample: BarcodedLibrary19_G_66h_DI

SAMN02928793 • SRS663503 • All experiments • All runs

Organism: Anopheles coluzzii

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted and DNase treated TruSeq® RNA Sample Preparation kit v2 (Illumina) for construction of RNA libraries (including a polyA-selection step), following manufacturer's protocols

Experiment attributes:

GEO Accession: GSM1440949

Links:

External link: GEO Sample GSM1440949

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 10.6M spots, 1.1G bases, 780.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR1521764	10,596,461	1.1G	780.5Mb	2016-03-09

ID:: 922791

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Sequence Read Archive

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