GSM1440948: BarcodedLibrary18_G_66h_DI; Anopheles coluzzii; RNA-Seq - SRA

SRX658350: GSM1440948: BarcodedLibrary18_G_66h_DI; Anopheles coluzzii; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 8.3M spots, 899M bases, 580.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

PRJNA255780 • SRP044683 • All experiments • All runs

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We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus. Overall design: After rearing in freshwater, both young and old larval instars of each species were briefly (6 h) exposed to either saltwater (SW) or freshwater (FW) conditions to test the impact of salinity on mRNA levels.

Sample: BarcodedLibrary18_G_66h_DI

SAMN02928792 • SRS663502 • All experiments • All runs

Organism: Anopheles coluzzii

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted and DNase treated TruSeq® RNA Sample Preparation kit v2 (Illumina) for construction of RNA libraries (including a polyA-selection step), following manufacturer's protocols

Experiment attributes:

GEO Accession: GSM1440948

Links:

External link: GEO Sample GSM1440948

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 8.3M spots, 899M bases, 580.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR1521763	8,323,916	899M	580.8Mb	2016-03-09

ID:: 922790

SRA

Sequence Read Archive

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