RNA-Seq of Hermertia illucens L: early stage egg - SRA

SRX5060463: RNA-Seq of Hermertia illucens L: early stage egg
1 ILLUMINA (Illumina HiSeq 2000) run: 36.5M spots, 11G bases, 4Gb downloads

Design: After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeqXTen.

Submitted by: Huazhong Agricultural University

Study: Transcriptome sequencing of black soldier fly

PRJNA506627 • SRP170683 • All experiments • All runs

show Abstracthide Abstract

investigate the fat accumulation mechanism by transcriptome sequencing.

Sample: collected in early egg stage

SAMN10465361 • SRS4076404 • All experiments • All runs

Organism: Hermetia illucens

Library:

Name: E-egg_1

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq