RNA-Seq of D. mojavensis from Las Bocas:adult male exposed to cactus ... - SRA

SRX4925204: RNA-Seq of D. mojavensis from Las Bocas:adult male exposed to cactus diet
1 ILLUMINA (Illumina HiSeq 2000) run: 5.1M spots, 516.6M bases, 273Mb downloads

Design: Newly emerged adult males from cactus diet were washed twice with distilled water and placed into 1.5ml tubes, then rinsed twice with 1X PBS. All liquid was then removed, and flies were frozen (-70oC) until RNA extraction. Frozen samples were homogenized with Tri-Reagent using Teflon homogenizers and extracted using Direct-zolTM RNA MiniPrep extraction kit (Zymo Research) according to the manufacturer's protocol. Libraries were prepared with TruSeq RNA Sample Preparation Kit v2 (Illumina), selecting only polyA mRNAs and synthezing double stranded cDNAs to attach to the Illumina adapters. Library size and quality were measure by Bioanalyzer (Agilent Technologies) and sequenced in a 1 X 100 single-end read format on a HiSeqTM 2000 Sequencing System (Illumina).

Submitted by: CINVESTAV

Study: Transcriptional responses to seasonal dietary shifts in Drosophila mojavensis

PRJNA498102 • SRP166549 • All experiments • All runs

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Drosophila mojavensis normally breeds in necrotic columnar cactus, but they also feed and breed in Opuntia fruit (prickly pear) which serves as a seasonal resource. The prickly pear fruits are much different chemically from cacti, mainly in their free sugars and lipid content, raising the question of the effects of this seasonal shift on gene expression. We evaluated gene expression of emerging male and female adult flies exposed to prickly pear fruits or cacti using RNA-Seq.

Sample: Individuals exposed to seminatural cactus-diets since second instar larva

SAMN10282801 • SRS3970468 • All experiments • All runs

Organism: Drosophila mojavensis

Library:

Name: SE_TAM_Dmoj_PPFvsCAC_S06

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Runs: 1 run, 5.1M spots, 516.6M bases, 273Mb

Run	# of Spots	# of Bases	Size	Published
SRR8098401	5,114,838	516.6M	273Mb	2019-11-01

ID:: 6626564

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