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SRX4115026: RNA-Seq of switchgrass: seedling
1 ILLUMINA (Illumina HiSeq 2000) run: 32.9M spots, 5.9G bases, 4Gb downloads

Design: After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide.
Submitted by: Northwest A&F University
Study: Slow seedling establishment of switchgrass
show Abstracthide Abstract
Transcriptomes from rapidly established (re) and slowly established (se) seedlings of switchgrass were sequenced to identify candidate genes involved in seedling establishment.
Sample:
SAMN09241274 • SRS3330437 • All experiments • All runs
Library:
Name: L9
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 32.9M spots, 5.9G bases, 4Gb
Run# of Spots# of BasesSizePublished
SRR720603632,923,0775.9G4Gb2019-08-06

ID:
5600587

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