CuR1 - SRA - NCBI

SRX3311455: CuR1
1 ILLUMINA (HiSeq X Ten) run: 86.7M spots, 26G bases, 10.7Gb downloads

Design: Total RNA was extracted from tissues using a TaKaRaMiniBEST PlantRNA Extraction Kit. During total RNA extraction, genomic DNA was removed by the DNase I treatment.Then RNA was analyzed either using a ND-8000spectrophotometer (NanodropTechnologies, Inc., Wilmington, DE, USA) after purification, by agarose gelelectrophoresis, or using a2100-Bioanalyzer(AgilentTechnologies, Santa Clara, CA, USA) to determine the RNA quantity. Those RNA samples with no smear seen on agarose gels, a 260/280 ratio above 2.0, and a RNA integrity number greater than 8.0 were used. For RNA-sequencing, RNA samples (three biological replicates per conditionand four plants per replicate) were then sent to Genergy Biotechnology Corporation (http://www.genenergy.cn/) for sequencing. Total RNA was first depleted of rRNA using the Ribo-Zero rRNA removal kit (Plant Leaf and Plant Seed/Root kits; Epicentre), namely, 1 μg of total RNA from root samples was used as input for rRNAremoval. Sequencing libraries were generated using the TruSeq RNA sample prep kit (Illumina). The libraries were sequenced as 151-bp paired-end reads using Illumina HiSeq X ten according to the manufacturer's instructions.

Submitted by: College of Life Sciences, Nanjing Agricultural University (College of Life Sciences, Nanjing Agricultural Uni)

Study: Oryza sativa Transcriptome under micronutrient deficiency in root.

PRJNA403975 • SRP117202 • All experiments • All runs

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Identify mirocronutrient deficiency dys-regulated genes using high-throughput RNA sequencing of rice roots under different mineral deficient growth conditions (Fe-, Zn-, Cu- or Mn-deficient).

Sample:

SAMN07627072 • SRS2617710 • All experiments • All runs

Organism: Oryza sativa Japonica Group

Library:

Name: Ribo-Zero rRNA removal

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Spot descriptor:

1 forward152 reverse

Runs: 1 run, 86.7M spots, 26G bases, 10.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR6201539	86,725,008	26G	10.7Gb	2018-05-11

ID:: 4639196

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