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SRX306805: GSM1154903: OL09004198; Melitaea cinxia; RNA-Seq
3 ILLUMINA (Illumina HiSeq 2000) runs: 15.5M spots, 1.6G bases, 930.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Genomic variation and adaptation in fragmented landscapes
show Abstracthide Abstract
We have compared allelic and gene expression variation using individual-based RNA-seq data from four regional populations of the Glanville fritillary butterfly (Melitaea cinxia) in northern Europe. Two of the populations represent fragmented habitat and two continuous habitat. Based on sequence information, we constructed genealogy for four populations. Based on gene expression, we found 1841 genes to be differentially expressed between two different landscape types. Our results demonstrate genomic adaptations to living in fragmented landscapes, which are likely to be related to phenotypic life-history adaptations that have been documented for many species. Overall design: RNA-seq from thorax, 174 individuals from four populations.
Sample: OL09004198
SAMN02191773 • SRS445731 • All experiments • All runs
Organism: Melitaea cinxia
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from frozen butterfly thorax with Trizol (Life Technologies Corporation, CA, USA) followed by chloroform, phenol-chloroform-isoamylalcohol and second chloroform purification. RNA was precipitated with isopropanol and washed with ethanol. RNA quality was verified with NanoDrop measurement (Thermo Fischer Scientific Inc., MA, USA) and Bioanalyzer run (Agilent Technologies, CA, USA). An in-house strand-specific library construction protocol based on polyA-anchoring.
Experiment attributes:
GEO Accession: GSM1154903
Links:
External link:
Runs: 3 runs, 15.5M spots, 1.6G bases, 930.5Mb
Run# of Spots# of BasesSizePublished
SRR9022114,680,616472.7M295.7Mb2014-07-06
SRR9022124,121,525416.3M238.6Mb2014-07-06
SRR9022136,712,698678M396.1Mb2014-07-06

ID:
430403

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