arabidopisis_Col-0_rep_2(aos) - SRA

SRX306527: arabidopisis_Col-0_rep_2(aos)
1 ILLUMINA (Illumina Genome Analyzer II) run: 20.3M spots, 3G bases, 1.9Gb downloads

Design: For each biological replicate, midgut tissue from 12 individual larvae (reared on a given host genotype) was pooled. Total RNA was extracted and isolated using a Qiagen RNAeasy kit. RNA samples were assessed for quality on a Bioanalyzer. Libraries were prepared using the mRNA-Seq8 Sample Prep Kit (Illumina), according to the manufacturer’s instructions. Libraries were prepared using the mRNA-Seq8 Sample Prep Kit (Illumina), according to the manufacturer’s instructions. Briefly, polyA-containing mRNA was purified and fragmented to an average size of approximately 2-300 nucleotides. The resulting fragmented RNA was reverse transcribed with random primers, made double stranded, end repaired, and A-tailed allowing ligation of the Illumina adaptors. Library molecules were then enriched by limited rounds of amplification, purified and analyzed for quality and size on an Agilent Bioanalyzer. All libraries were sequenced with a 75 bp paired-end sequencing run performed on the Illumina Genome Analyzer II.

Submitted by: PLANT RESEARCH LABS

Study: Trichoplusia ni Transcriptome or Gene expression

PRJNA208207 • SRP025999 • All experiments • All runs

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Trichoplusia ni expression of genes in the midgut upon growth on different host plant genotypes.

Sample: Trichoplusia ni reared on Arabidopis thaliana wildytpe Col-0 replicate 2 (control for JA mutant experiment)

SAMN02202236 • SRS444412 • All experiments • All runs

Organism: Trichoplusia ni

Library:

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Spot descriptor:

1 forward76 reverse

Runs: 1 run, 20.3M spots, 3G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR901771	20,309,516	3G	1.9Gb	2014-06-12

ID:: 430125

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