Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using the RNeasy extraction kit (Qiagen, cat# 75142) following manufactures instructions that included an in-column DNAse digestion. Poly A RNA sselection. Quality of the RNA was then confirmed using Agilent 2100 Bioanalyzer. RNA library preparation and high-throughput sequencing were performed on an Illumina HiSeq 2000 sequencing system following the protocol recommended by Illumina for sequencing total RNA samples. Strand-specific library prep. Sequencing was done for each biological replicates at 1 x 75bp strand specific by the Yale Center for Genome Analysis