Genome sequencing of synthetic Escherichia coli, C321.ĆA.740 - SRA

SRX2511758: Genome sequencing of synthetic Escherichia coli, C321.ĆA.740
1 ILLUMINA (Illumina HiSeq 2000) run: 186,369 spots, 56.1M bases, 14.6Mb downloads

Design: gDNA prep. cell culture was processed with a Qiagen DNeasy Blood and Tissue (cat: 69504) to extract genomic DNA. Sequencing. 2.5 g of gDNA was sent to the Yale Center Genome Analysis for library prep. One micrograms of gDNA were sheared to an expected size of 500 bases with Covaris E210 in a covaris microtube. Post-shearing cleanup was done with SPRI magnetic beads. ÔWith BeadŐ fragment end repair was performed with End Repair enzyme at 20űC for 30 min and purified. ÔWith beadŐ A-base addition was performed with A-Tailing enzyme at 30_C for 30 min and purified. Samples were barcoded with an adapter ligation mix. Ligation was purified. Samples were PCR enriched (26-l DNA; 30-l KAPA HiFi Mastermix; 2-ul 25-M PCR Primer MP1.0; 2-l 25-M barcode-specific primer). Samples were loaded onto a lane of an Illumina HiSeq 2000 for 76-base pairedend reads.

Submitted by: Northwestern University

Study: synthetic Escherichia coli C321.deltaA Genome sequencing

PRJNA361365 • SRP097637 • All experiments • All runs

show Abstracthide Abstract

Here, we have sequenced the genomes of engineered strains of genomically recoded Escherichia coli lacking RF1 used to produce high yielding cell-free protein synthesis (CFPS) platform from crude extracts.

Sample: Escherichia coli C321.deltaA.740

SAMN06232395 • SRS1935451 • All experiments • All runs

Organism: Escherichia coli

Library:

Name: C321.ĆA.740

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 186,369 spots, 56.1M bases, 14.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR5196087	186,369	56.1M	14.6Mb	2017-01-24

ID:: 3638132

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