rice RNA structurome biological replicate 2 - SRA

SRX2214260: rice RNA structurome biological replicate 2
1 ILLUMINA (Illumina HiSeq 2500) run: 45M spots, 6.7G bases, 3.3Gb downloads

Design: The wild-type rice were grown in greenhouse and shoot tissue of two-week-old plant was used for library preparation. 1 g of shoot tissue were cut off the plant right before each treatment. After following our previous pipeline of Structure-seq for +DMS treatment, the -DMS sample was processed with no DMS applied as the control. For the heat parallel experiment, -DMS and +DMS samples were prepared. Library preparation was following our previous library construction pipeline with some major changes and optimization. All 12 individual biological libraries was done with unique Illumina TruSeq barcode and been sequenced by HiSeq2500.

Submitted by: The Pennsylvania State University

Study: Oryza sativa Raw sequence reads

PRJNA345449 • SRP090901 • All experiments • All runs

show Abstracthide Abstract

in vivo genome-wide RNA secondary structure profiling under heat stress in rice

Sample: Room temperature sample 5

SAMN05863940 • SRS1730548 • All experiments • All runs

Organism: Oryza sativa Japonica Group

Library:

Name: H

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq