GSM2285035: Sample_Matthews_SGM3; Cavia porcellus; RNA-Seq - SRA

SRX2035023: GSM2285035: Sample_Matthews_SGM3; Cavia porcellus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 49.6M spots, 2.5G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)

Study: The Transgenerational Effects of Prenatal Synthetic Glucocorticoids

PRJNA339489 • SRP082393 • All experiments • All runs

show Abstracthide Abstract

First generation animals (F1) were exposed to multiple courses of synthetic glucocorticoids in utero. Animals were then bred to produce second (F2) and third (F3) generation offspring. These animals underwent behavioural assessmenets before sacrifice. In this experiment the paraventricular nucleus of the hypothalamus fo F1, F2, and F3 female anmials (Beta and Control) have been extracted via micro-punch and RNA has been extracted. Overall design: F1 Control n=5 vs F1 Beta n=5, F2 Control n=5 vs F2 Beta n=5, F3 Control n=6 vs F3 Beta n=6

Sample: Sample_Matthews_SGM3

SAMN05591965 • SRS1628961 • All experiments • All runs

Organism: Cavia porcellus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Brains were removed, flash frozen on dry ice. Paranvetricular nucleus was extracted via micro-punch. RNA was harvested using AllPrep RNA/DNA/microRNA Universal Extraction Kit (Qiagen Cat#80224).TruSeq RNA Library Preparation Kit V2 (Cat#RS-122-2001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2285035

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 49.6M spots, 2.5G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR4044118	49,605,257	2.5G	1.1Gb	2017-11-15

ID:: 2942488

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Sequence Read Archive

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