RNA-seq of Mirounga angustirostris: blubber - SRA

SRX1713227: RNA-seq of Mirounga angustirostris: blubber
1 ILLUMINA (Illumina HiSeq 2500) run: 15.9M spots, 3.2G bases, 1.7Gb downloads

Design: Libraries for sequencing were prepared according to TruSeq protocol (TruSeq RNA Sample Prep Kit v2, Illumina, USA). Specifically, mRNA was isolated from total RNA samples using oligo-dT magnetic beads, fragmented and used as template for first-strand cDNA synthesis with random hexamers (SuperScript II Reverse Transcriptase, Invitrogen, USA). After double-stranded (ds) cDNA synthesis and cDNA purification, overhang fragments were end-repaired. This was followed by adenylation of 3_ ends of the blunt-ended fragments and ligation to proprietary adapter oligonucleotides (Illumina, USA). Ligation products were subjected to PCR amplification (10-12 cycles) before library quantification and validation (DNA High Sensitivity Assay, Agilent Technologies, USA).

Submitted by: Sonoma State University

Study: Mirounga angustirostris strain:northern elephant seal Random survey

PRJNA258305 • SRP045540 • All experiments • All runs

show Abstracthide Abstract

Muscle tissue gene expression profile in juvenile northern elephant seals during an acute stress challenge

Sample: SB3-T0_CGATGT_L004

SAMN04595358 • SRS1402545 • All experiments • All runs

Organism: Mirounga angustirostris

Library:

Name: SB3-T0

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq