Heterocephalus glaber skin stranded RNA-seq - SRA

SRX1113910: Heterocephalus glaber skin stranded RNA-seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 35.4M spots, 14.2G bases, 8.3Gb downloads

Design: RNA was isolated using the RNeasy Mini kit (Qiagen) including proteinase digest of the tissue. The RNA was treated with DNase I on the affinity column before elution. Strand-specific RNA-seq libraries, including poly-A(+) mRNA selection and RNA fragmentation, were prepared using the TruSeq Stranded RNA LT Kit (Illumina). The resulting libraries had insert sizes of ca. 100-400 bp as indicated by DNA 7500 Chips run on an Agilent Bioanalyzer 2100 instrument (Agilent). Ten libraries were combined into a single pool for sequencing.

Submitted by: Fritz Lipmann Institute, Institute for Ageing Rese

Study: Heterocephalus glaber Transcriptome or Gene expression

PRJNA283581 • SRP061363 • All experiments • All runs

show Abstracthide Abstract

Illumina RNA-seq of the naked mole-rat using strand-specific libraries. Sequencing of diverse inner organ samples in order to obtain a transcript catalog of Heterocephalus glaber.

Sample:

SAMN03653044 • SRS1008276 • All experiments • All runs

Organism: Heterocephalus glaber

Library:

Name: Hetgla skin

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward201 reverse

Runs: 2 runs, 35.4M spots, 14.2G bases, 8.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR2120844	17,790,039	7.1G	4.2Gb	2015-07-31
SRR2120910	17,589,032	7G	4.1Gb	2015-07-31

ID:: 1625915

SRA

Sequence Read Archive

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