transcriptome of TGMS SP2S at 16 Celsius degree - SRA

SRX1049907: transcriptome of TGMS SP2S at 16 Celsius degree
1 ILLUMINA (Illumina HiSeq 2000) run: 23M spots, 4.6G bases, 3Gb downloads

Design: Firstly, young floral buds from 4 treatments including FL (SP2F at 16°C), SL (SP2S at 16°C), FR (SP2F at 22°C), and SR (SP2S at 22°C) were collected, put into RNAlater, and stored at −80°C until further processing. Total RNAs were extracted using the Trizol® Reagent (Invitrogen, USA according to the manufacturer's instructions, and purified using TRK1001 Kit (LC Science, Houston, TX). The total RNA quantity and purity were analyszed by using of a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA). We used 10 µg total RNA from each sample to create normalized cDNAs. The cDNAs were amplified according to the Illumina RNA-Seq protocol and sequenced on Illumina Hiseq2000 platform. Raw data generated from Solexa were trimmed to remove nonsense sequences including adaptor, low-quality reads, and very short sequences.

Submitted by: Northwest A&F University

Study: Brassica napus breed:SP2S Transcriptome or Gene expression

PRJNA285685 • SRP058985 • All experiments • All runs

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A comparative transcriptome analysis of SP2S flower bud and its Near isogenic line SP2F was conducted

Sample: flower buds from TGMS under low temperature

SAMN03761887 • SRS954134 • All experiments • All runs

Organism: Brassica napus

Library:

Name: SP2 cool

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 23M spots, 4.6G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR2052475	22,954,338	4.6G	3Gb	2016-06-04

ID:: 1524035

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