Altered gene expression associated with a thermo-sensitive male steri... - SRA

SRX1046595: Altered gene expression associated with a thermo-sensitive male sterility mutant SP2S in rapeseed (Brassica napus)
1 ILLUMINA (Illumina HiSeq 2000) run: 86.1M spots, 17.4G bases, 11.3Gb downloads

Design: Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA)

Submitted by: Northwest A&F University

Study: Brassica napus breed:SP2S Transcriptome or Gene expression

PRJNA285685 • SRP058985 • All experiments • All runs

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A comparative transcriptome analysis of SP2S flower bud and its Near isogenic line SP2F was conducted

Sample: Brassica napus flower buds from temperature-sensitive male sterile line SP2S

SAMN03757452 • SRS951359 • All experiments • All runs

Organism: Brassica napus

Library:

Name: SP2S

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 86.1M spots, 17.4G bases, 11.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR2048494	86,075,091	17.4G	11.3Gb	2016-06-02

ID:: 1519652

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