SRX017282: Anopheles gambiae gastric caeca transcriptome
1 LS454 (454 GS FLX) run: 218,732 spots, 37.7M bases, 92Mb downloads
Design: Total RNA is extracted from 10 Gastric Caeca of Anopheles gambiae. Library construction starts with total RNA reverse transcribed to cDNA with an oligo dT primer, with second strand then generated. The cDNA is digested with a restriction enzyme then adaptors ligated onto the double-stranded (ds) DNA. The adaptor ligated cDNA fragments are the amplified by PCR. This product is purified and processed for emPCR and 454 sequencing according to manufactures procedures. Adaptors for library construction: 5' targeted library A 5' GCCTCCCTCGCGCCATCAG 3' B 5' GCCTTGCCAGCCCGCTCAG 3' 3' targeted library A 5'-CCATCTCATCCCTGCGTGTCCCATCTGTTCCCTCCCTGTCTCAG-3' B 5'-CCTATCCCCTGTGTGCCTTGCCTATCCCCGCAGTCGGTACTTTT-3'
Submitted by: University of Florida - Leonid Moroz (UF-LM)
Study:
Anopheles gambiae transcriptomeshow Abstracthide AbstractTranscritome analysis from the Gastric Caeca and Malpighian tubules of Anopheles gambiae . Total RNA was extracted from 10 animals. cDNA librarie were generated and sequenced using a 454 platform.
Sample:
Anopheles gambiae M gastric caecaLibrary:
Name: Gastric Caeca
Instrument: 454 GS FLX
Strategy: AMPLICON
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: SINGLE
Spot descriptor:
5 forward
Pipeline:
show...hide...| Program |
|---|
| 454 Basecaller Base Caller |
| 454 Basecaller Quality Scorer |
Runs:
1 run, 218,732 spots, 37.7M bases, 92Mb