Ribosome profiling of HEK293 cells treated with DMSO - SRA

SRX205666: Ribosome profiling of HEK293 cells treated with DMSO
2 ILLUMINA (Illumina HiSeq 2000) runs: 14.4M spots, 689.9M bases, 392.3Mb downloads

Design: Ribosomes were isolated from cell lysates using sucrose gradient and ultracentrifugation. Ribosome fractions were combined and digested by RNase I. Then, RNAs were extracted and ribosome protected mRNA fragments were size selected and purified. Next, framents were added with Poly-(A) tails and converted to cDNAs by reverse transcription. Afterwards, cDNAs were circulated and re-linealized. Finally, cDNAs were PCR amplified and library fragments were isolated.

Submitted by: CORNELL UNIVERSITY (CORNELL)

Study: Co-Translational Response to Proteotoxic Stress by Elongation Pausing of Ribosomes

PRJNA181016 • SRP017263 • All experiments • All runs

show Abstracthide Abstract

Translational control permits cells to respond swiftly to changing environment. Rapid attenuation of global protein synthesis under stress conditions has been largely ascribed to the inhibition of translation initiation. To monitor the co-translational response to proteotoxic stress especially during elongation stage, we performed ribosome profiling (deep sequencing of ribosome-protected mRNA fragments) and investigated the roles of chaperone molecules in this process.

Sample: HEK293_DMSO

SAMN01816434 • SRS376440 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 2 runs, 14.4M spots, 689.9M bases, 392.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR619088	10,086,263	484.1M	269.8Mb	2013-01-02
SRR619089	4,286,560	205.8M	122.5Mb	2013-01-02

ID:: 278188

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