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SRX195697: GSM1020243: Footprints from cells infected with HCMV for 72hr, no drug pretreatment; Human herpesvirus 5; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 20.7M spots, 826.9M bases, 546Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Decoding human cytomegalovirus using ribosome profiling
show Abstracthide Abstract
The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like other complex viruses, our understanding of its protein coding potential is far from complete. Here, we use ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified several hundred previously unidentified open reading frames and confirmed a fraction by mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity. Overall design: Ribosome profiling and mRNA-seq from 3 time points (5hr, 24hr, 72hr) along HCMV infection. The supplementary file 'GSE41605_merlin_final_orfs.bed.gz' includes the 751 ORFs of HCMV that were identified in this study.
Sample: Footprints from cells infected with HCMV for 72hr, no drug pretreatment
SAMN01766548 • SRS369476 • All experiments • All runs
Library:
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Experiment attributes:
GEO Accession: GSM1020243
Links:
External link:
Runs: 1 run, 20.7M spots, 826.9M bases, 546Mb
Run# of Spots# of BasesSizePublished
SRR59296220,673,613826.9M546Mb2015-07-22

ID:
259483

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